Persistence of Borrelia burgdorferi Following Antibiotic Treatment in Mice Antimicrobial Agents and Chemotherapy,

 Persistence of Borrelia burgdorferi Following Antibiotic Treatment in Mice 
Antimicrobial Agents and Chemotherapy, published online ahead of print on 3 March 2008 
Emir Hodzic, Sunlian Feng, Kevin Holden, Kimberly J. Freet, and Stephen W. Barthold
The effectiveness of antibiotic treatment was examined in a mouse model of Lyme borreliosis.
Mice were treated with ceftriaxone or saline for one month, commencing during the early, 3 
weeks, or chronic, 4 months, stages of infectionwith Borrelia burgdorferi. Tissues from mice 
were tested for infection by culture, polymerase chain reaction, PCR, xenodiagnosis, and 
transplantation of allografts at 1 and 3 months after completion of treatment. In addition, tissues 
were examined for spirochetes by immunohistochemistry.
In contrast to saline-treated mice, mice treated with antibiotic were consistently 
culture-negative, but tissues from some of the mice remained PCR-positive, and spirochetes 
could be visualized in collagen-rich tissues. Furthermore, when some of the antibiotic treated 
mice were fed upon by Ixodes scapularis ticks, xenodiagnosis, spirochetes were acquired by the 
ticks, based upon PCR, and ticks from those cohorts transmitted spirochetes to naïve SCID mice, 
which became PCR-positive, but culture-negative.
Results indicated that following antibiotic treatment, mice remained infected with 
non-dividing but infectious spirochetes, particularly when antibiotic treatment was commenced 
during the chronic stage of infection




Share This
Pin on PinterestShare on FacebookTweet about this on TwitterShare on StumbleUponShare on Google+

Leave a Reply

Your email address will not be published.