Thomas Grier, M.S. Fundraiser for Research Equipment

From Thomas Grier:

“I am setting up a lab to do basic research and also supportive work for practitioners to help support their diagnosis.

Research project I want to do: * Brain pathology on Deer brains to determine if they have Borrelia infections in the brain.

* Transmission from tick to rabbit in less than 24 hours by improperly removing ticks,

*Time and Temperature studies on Borrelia to determine lethal temperatures and the effectiveness of using heat as an adjunct therapy to antibiotic and alternative treatments.”

Order of importance of equipment:

A) a compound Light microscope with darkfiled condensor and video camera $800

B) a dissection scope for field work on mice and ticks $ 500

C) Slides, slip covers, stains, petri dishes, glassware, $500

D) Temperature controlled Incubation cabinet for culture and temperature studies and a tissue dehydrator for paraffin fixing brain tissue (Used can vary from $200-1,000 )

E) Deep Freeze tissue freezer $400

F) Centrifuge $ 300

Estimated cost is less than $ 3,500

Microscopes and equipment that would really be nice would be a fluorescent scope $2500, and a PCR thermal multiplier to help determine Borrelia species by PCR – $2,000

These are two different scopes for different purposes.

The SM2tZZ is a dissection scope. This scope is where tissues have to be prepped and wild mice have to be dissected for animal studies and to obtain the WILD Strains of Bb. This microscope observes and enlarges the sample like a fancy magnifying glass. This type of scope is best as a ZOOM, fixed lenses are a hassle. This is the kind of scope used in biology class to look at things like bird feathers.

The T490 is a compound microscope for doing pathology work and whole blood analysis. This microscope looks through the sample. The sample has to be a single cell layer thick. The T490 series has much better lenses than the previous 400 series scopes.

Other dream scopes are the fluorescent scopes for $2,500 which would be an incredible research tool for live research, and the best is the confocal laser fluorescent scope for $10,000, which can observe live cells and make 3-D images.

Here is some basic information on who Thomas Grier, M.S. is:

Click here to see how Tom got involved in Lyme Research:
http://sfec.cfans.umn.edu/prod/groups/cfans/@pub/@cfans/@sfec/documents/article/cfans_article_422768.pdf

Tom’s Personal Lyme Story
http://www.mdjunction.com/forums/lyme-disease-support-forums/studies-research/374434-tom-griers-lyme-ms-story

Why do we call them experts, by Tom Grier
http://whatislyme.com/why-do-we-call-them-experts-by-tom-grier/

Who will be the heroes of Lyme Disease, who will be the villians? by Thomas Grier, M.S.
http://whatislyme.com/who-will-be-the-heroes-of-lyme-disease-who-will-be-the-villains/

Lyme on the Brain
http://www.mdjunction.com/lyme-disease/articles/lyme-on-the-brain-by-tom-grier-part-1-of-4-august-5-2010

Motile Menace
http://www.lymeneteurope.org/info/motile-menace

The Difficulty in Culturing Spirochetes
http://www.lymeneteurope.org/info/the-difficulty-of-culturing-spirochetes

Chronic Lyme Post-Mortem Study Needed
http://www.lymeneteurope.org/info/chronic-lyme-post-mortem-study-needed

DEET vs. Permethrin as a Tick Repellent
http://www.lymeneteurope.org/info/deet-versus-permethrin-as-a-tick-repellent

Why The Controversy Over Diagnosing and Treating Lyme Disease
By Tom Grier
(As published in the Daily Reader newspaper, Ashland, Wisconsin)
http://www.lymeneteurope.org/info/why-the-controversy-over-diagnosing-and-treating-lyme-disease

Notes and Observations on Cell Wall Deficient Forms
http://www.lymeneteurope.org/info/notes-and-observations-on-cell-wall-deficient-forms

Why Are We Still Sick
http://www.lymeneteurope.org/info/why-are-we-still-sick





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